Loss of RNF43 Function Contributes to Gastric Carcinogenesis by Impairing DNA Damage Response.

ABSTRACT:

Background & aims

RING finger protein 43 (RNF43) is a tumor suppressor that frequently is mutated in gastric tumors. The link between RNF43 and modulation of Wingless-related integration site (WNT) signaling has not been shown clearly in the stomach. Because mutations in RNF43 are highly enriched in microsatellite-unstable gastric tumors, which show defects in DNA damage response (DDR), we investigated whether RNF43 is involved in DDR in the stomach.

Methods

DDR activation and cell viability upon γ-radiation was analyzed in gastric cells where expression of RNF43 was depleted. Response to chemotherapeutic agents 5-fluorouracil and cisplatin was analyzed in gastric cancer cell lines and xenograft tumors. In addition, involvement of RNF43 in DDR activation was analyzed upon He

SUBMITTER: Neumeyer V 

PROVIDER: S-EPMC7898035 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature