Propionate Enhances Cell Speed and Persistence to Promote Intestinal Epithelial Turnover and Repair.

ABSTRACT:

Background and aims

Gut bacteria-derived short-chain fatty acids (SCFAs) play crucial roles in the maintenance of intestinal homeostasis. However, how SCFAs regulate epithelial turnover and tissue repair remain incompletely understood. In this study, we investigated how the SCFA propionate regulates cell migration to promote epithelial renewal and repair.

Methods

Mouse small intestinal epithelial cells (MSIE) and human Caco-2 cells were used to determine the effects of SCFAs on gene expression, proliferation, migration, and cell spreading in vitro. Video microscopy and single cell tracking were used to assess cell migration kinetically. 5-bromo-2'-deoxyuridine (BrdU) and hydroxyurea were used to assess the effects of SCFAs on migration in vivo. Lastly, an acute colitis model

SUBMITTER: Bilotta AJ 

PROVIDER: S-EPMC7898181 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature