Association of human papillomavirus integration with better patient outcomes in oropharyngeal squamous cell carcinoma.

ABSTRACT:

Background

The molecular drivers of human papillomavirus-related head and neck squamous cell carcinoma (HPV + HNSCC) are not entirely understood. This study evaluated the relationship between HPV integration, expression of E6/E7, and patient outcomes in p16+ HNSCCs.

Methods

HPV type was determined by HPV PCR-MassArray, and integration was called using detection of integrated papillomavirus sequences polymerase chain reaction (PCR). We investigated whether fusion transcripts were produced by reverse transcriptase polymerase chain reaction (RT-PCR). E6/E7 expression was assessed by quantitative RT-PCR. We assessed if there was a relationship between integration and E6/E7 expression, clinical variables, or patient outcomes.

Results

Most samples demonstrated HPV integrati

SUBMITTER: Pinatti LM 

PROVIDER: S-EPMC7898416 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature