Microbial yield from infectious tissues pretreated by various methods: an invitro study.

ABSTRACT:

Background

This study aimed to evaluate the effects of different pretreatment methods on the microbial yield from infectious tissues.

Methods

Strains of Staphylococcus aureus (SA), Escherichia coli (EC) and Candida albicans (CA) were used to construct single-surface, full-surface, and internal infection models in sterile pork tissue. Manual milling (MM), mechanical homogenization (MH), sonificated (SF), dithiothreitol (DTT), and direct culture (DC) were used to pretreat these tissues, the microbial yield from different pretreatment methods were recorded and compared. Moreover, periprosthetic tissues collected intraoperatively from periprosthetic joint infection (PJI) patients were used as a verification.

Results

The study showed that the microbial yield from MH pretre

SUBMITTER: Cai Y 

PROVIDER: S-EPMC7898421 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature