Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer's disease.

ABSTRACT:

Background

High-density oligomers of the prion protein (HDPs) have previously been identified in brain tissues of patients with rapidly progressive Alzheimer's disease (rpAD). The current investigation aims at identifying interacting partners of HDPs in the rpAD brains to unravel the pathological involvement of HDPs in the rapid progression.

Methods

HDPs from the frontal cortex tissues of rpAD brains were isolated using sucrose density gradient centrifugation. Proteins interacting with HDPs were identified by co-immunoprecipitation coupled with mass spectrometry. Further verifications were carried out using proteomic tools, immunoblotting, and confocal laser scanning microscopy.

Results

We identified rpAD-specific HDP-interactors, including the growth arrest specific

SUBMITTER: Shafiq M 

PROVIDER: S-EPMC7898440 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature