Successful 18-h acellular extracorporeal perfusion and replantation of porcine limbs - Histology versus nerve stimulation.

ABSTRACT: The current standard for composite tissue preservation is static cold storage (SCS) and is limited to 6 h until irreversible muscle damage occurs. Extracorporeal perfusion (ECP) is a promising technique for prolonged preservation, however, functional results have been scarcely researched. This article assessed neuromuscular function and compared results to histological alterations to predict muscle damage after ECP. Forelimbs of twelve Dutch landrace pigs were amputated and preserved by 4 h SCS at 4-6 °C (n = 6) or 18 h mid-thermic ECP with University of Wisconsin solution (n = 6). Limbs were replanted and observed for 12 h. Sham surgery was performed on contralateral forelimbs (n = 12). Histology analysis scored four subgroups representing different alterations (higher score equals more d

SUBMITTER: Kruit AS 

PROVIDER: S-EPMC7898521 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature