Synthesis and Evaluation of Non-Hydrolyzable Phospho-Lysine Peptide Mimics.

ABSTRACT: The intrinsic lability of the phosphoramidate P-N bond in phosphorylated histidine (pHis), arginine (pHis) and lysine (pLys) residues is a significant challenge for the investigation of these post-translational modifications (PTMs), which gained attention rather recently. While stable mimics of pHis and pArg have contributed to study protein substrate interactions or to generate antibodies for enrichment as well as detection, no such analogue has been reported yet for pLys. This work reports the synthesis and evaluation of two pLys mimics, a phosphonate and a phosphate derivative, which can easily be incorporated into peptides using standard fluorenyl-methyloxycarbonyl- (Fmoc-)based solid-phase peptide synthesis (SPPS). In order to compare the biophysical properties of natural pLys with ou

SUBMITTER: Hauser A 

PROVIDER: S-EPMC7898648 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature