Cancer-derived exosomal miR-7641 promotes breast cancer progression and metastasis.

ABSTRACT:

Background

Intercellular communication is crucial for breast cancer progression and metastasis. However, the role of cancer-derived exosomes and their crucial microRNA (miRNA) cargoes mediating intercellular communication requires further investigation.

Methods

Cancer-derived exosomes were isolated using differential centrifugation and differentially expressed miRNAs were determined by microarrays and qRT-PCR analysis. Cell proliferation, wound-healing, Transwell invasion, and tumor xenograft assays were used for functional research. Plasma exosomal RNA was isolated to verify its role as a prognostic biomarker.

Results

We found that the tumor-promoting capacity of the exosomes was positively related to their cells of origin. MiR-7641 was identified to be the most diff

SUBMITTER: Shen S 

PROVIDER: S-EPMC7898766 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature