Loss of SIRT1 in diabetes accelerates DNA damage-induced vascular calcification.

ABSTRACT:

Aims

Vascular calcification is a recognized predictor of cardiovascular risk in the diabetic patient, with DNA damage and accelerated senescence linked to oxidative stress-associated pathological calcification. Having previously shown that systemic SIRT1 is reduced in diabetes, the aim was to establish whether SIRT1 is protective against a DNA damage-induced senescent and calcified phenotype in diabetic vascular smooth muscle cells (vSMCs).

Methods and results

Immunohistochemistry revealed decreased SIRT1 and increased DNA damage marker expression in diabetic calcified arteries compared to non-diabetic and non-calcified controls, strengthened by findings that vSMCs isolated from diabetic patients show elevated DNA damage and senescence, assessed by the Comet assay and telome

SUBMITTER: Bartoli-Leonard F 

PROVIDER: S-EPMC7898956 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature