Quantitative analysis of RNA by HPLC and evaluation of RT-dPCR for coronavirus RNA quantification.

ABSTRACT: Nucleic acid detection and quantification have been known to be important at various fields, from genetically modified organisms and gene expression to virus detection. For DNA molecules, digital PCR has been developed as an absolute quantification method which is not dependent on external calibrators. While when it comes to RNA molecules, reverse transcription (RT) step must be taken before PCR amplification to obtain cDNA. With different kinds of reverse transcriptase (RTase) and RT reaction conditions being used in laboratory assays, the efficiency of RT process differs a lot which led variety in quantification results of RNA molecules. In this study, we developed HPLC method combined with enzymatic digestion of RNA to nucleotides for quantification of RNA without RT process. This metho

SUBMITTER: Niu C 

PROVIDER: S-EPMC7898971 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature