Two approaches for the stabilization of Bacillus anthracis recombinant protective antigen.

ABSTRACT: Anthrax is a zoonotic disease caused by the gram-positive spore-forming bacteria Bacillus anthracis. There is a need for safe, highly effective, long-term storage vaccine formulations for mass vaccination. However, the development of new subunit vaccines based on recombinant protective antigen (rPA) faces the problem of vaccine antigen instability. Here, the potential of simultaneous application of two different approaches to stabilize rPA was demonstrated. Firstly, we employed spherical particles (SPs) obtained from the tobacco mosaic virus (TMV). Previously, we had reported that SPs can serve as an adjuvant and platform for antigen presentation. In the current work, SPs were shown to increase the stability of the full-size rPA without loss of its antigenic properties. The second d

SUBMITTER: Ryabchevskaya EM 

PROVIDER: S-EPMC7899644 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature