Epithelial Regeneration in Human Corneas Preserved in an Active Storage Machine.

ABSTRACT:

Purpose

To characterize the corneal epithelium (CE) and limbal epithelium (LE) of human corneas stored in an innovative active storage machine (ASM) after a period of organ culture (OC).

Methods

Corneas unsuitable for graft and stored in a standard commercial OC medium for 2 to 5 weeks were transferred into our ASM for 14 days. The ASM actively maintained an overpressure on the endothelial side (20 mm Hg) while ensuring medium renewal. We compared three modalities of storage in the ASM's epithelial chamber: (1) alternating exposure to a supplemental hormonal epithelial medium (SHEM) and air (air-lifting), (2) continuous immersion in SHEM, and (3) continuous immersion in OC medium. Passive immersion of the whole cornea in OC medium or of the CE in SHEM with or without airlift

SUBMITTER: Guindolet D 

PROVIDER: S-EPMC7900847 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature