EMC3 Is Essential for Retinal Organization and Neurogenesis During Mouse Retinal Development.

ABSTRACT:

Purpose

We used a mouse model to explore the role of the endoplasmic reticulum membrane protein complex subunit 3 (EMC3) in mammalian retinal development.

Methods

The transcription pattern of Emc3 in C57BL/6 mice was analyzed by in situ hybridization. To explore the effects of EMC3 absence on retinal development, the Cre-loxP system was used to generate retina-specific Emc3 in knockout mice (Emc3flox/flox, Six3-cre+; CKO). Morphological changes in the retina of E13.5, E17.5, P0.5, and P7 mice were observed via hematoxylin and eosin staining. Immunofluorescence staining was used to assess protein distribution and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining to assess apoptosis changes. Proteins were identified and quantified by Western blottin

SUBMITTER: Cao X 

PROVIDER: S-EPMC7900856 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature