Cytoskeletal Proteins in Myotendinous Junctions of Human Extraocular Muscles.

ABSTRACT:

Purpose

The purpose of this study was to investigate the cytoskeletal composition of myotendinous junctions (MTJs) in the human extraocular muscles (EOMs). Desmin and other major cytoskeletal proteins are enriched at the MTJs of ordinary myofibers, where they are proposed to be of particular importance for force transmission and required to maintain myofiber integrity.

Methods

EOM and limb muscle samples were analyzed with immunohistochemistry using antibodies against the intermediate filament proteins desmin, nestin, keratin 19, vimentin, and different myosin heavy chain (MyHC) isoforms. MTJs were identified by labeling with antibodies against laminin or tenascin.

Results

In contrast to MTJs in lumbrical muscle where desmin, nestin, and keratin 19 were always present

SUBMITTER: Liu JX 

PROVIDER: S-EPMC7900863 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature