Early phase dynamics of traceable mCherry fluorescent protein-carrying HIV-1 infection in human peripheral blood mononuclear cells-transplanted NOD/SCID/Jak3^-/- mice.

ABSTRACT: We attempted to elucidate early-phase dynamics of HIV-1 infection using replication-competent, red-fluorescent-protein (mCherry)-labeled HIV-1_JR-FL (HIV_JR-FL^mC) and NOD/SCID/Jak3^-/- mice transplanted with Individual-A's human peripheral blood mononuclear cells (hPBMC)(hNOJ mice). On day 7 following HIV_JR-FL^mC inoculation, mCherry-signal-emitting infection foci were readily identified in the subserosa of 10 of 10 HIV_JR-FL^mC-inoculated hNOJ mice, although infection foci were not located without the mCherry signal in unlabeled HIV-1_JR-FL-inoculated mice (n = 6). Even on day 14, infection foci were hardly located in the unlabeled HIV-1_JR-FL-inoculated mice, while in all of 7 HIV_JR

SUBMITTER: Higashi-Kuwata N 

PROVIDER: S-EPMC7900919 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature