Optimization of protoplast regeneration in the model plant Arabidopsis thaliana.

ABSTRACT:

Background

Plants have a remarkable reprogramming potential, which facilitates plant regeneration, especially from a single cell. Protoplasts have the ability to form a cell wall and undergo cell division, allowing whole plant regeneration. With the growing need for protoplast regeneration in genetic engineering and genome editing, fundamental studies that enhance our understanding of cell cycle re-entry, pluripotency acquisition, and de novo tissue regeneration are essential. To conduct these studies, a reproducible and efficient protoplast regeneration method using model plants is necessary.

Results

Here, we optimized cell and tissue culture methods for improving protoplast regeneration efficiency in Arabidopsis thaliana. Protoplasts were isolated from whole seedlings of f

SUBMITTER: Jeong YY 

PROVIDER: S-EPMC7901198 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature