Methodology for evaluating and comparing flow cytometers: A multisite study of 23 instruments.

ABSTRACT: We demonstrate improved methods for making valid and accurate comparisons of fluorescence measurement capabilities among instruments tested at different sites and times. We designed a suite of measurements and automated data processing methods to obtain consistent objective results and applied them to a selection of 23 instruments at nine sites to provide a range of instruments as well as multiple instances of similar instruments. As far as we know, this study represents the most accurate methods and results so far demonstrated for this purpose. The first component of the study reporting improved methods for photoelectron scale (Spe) evaluations, which was published previously (Parks, El Khettabi, Chase, Hoffman, Perfetto, Spidlen, Wood, Moore, and Brinkman: Cytometry A 91 (2017) 232-249).

SUBMITTER: Parks DR 

PROVIDER: S-EPMC7901711 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature