A method for colocalizing lineage tracing reporter and RNAscope signals on skeletal tissue section.

ABSTRACT: Fluorescent reporters have been widely used in modern biology as a powerful tool in cell lineage tracing during development and in studying the pathogenesis of diseases. RNAscope is a recently developed RNA in situ hybridization method with high specificity and sensitivity. Combined application of these two techniques on skeletal tissue is difficult and has not been done before; the reporter fluorophores in the tissue specimen bleach quickly and mRNAs degrade rapidly due to the decalcification process typically used in processing skeletal samples. Therefore, we developed a method that can simultaneously detect and colocalize both the fluorescent lineage tracing reporter signal and the RNAscope signal in the same skeletal section without compromising the fidelity, sensitivity, and specifici

SUBMITTER: Yang H 

PROVIDER: S-EPMC7901837 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature