Expression of NanoLuc Luciferase in Listeria innocua for Development of Biofilm Assay.

ABSTRACT: Studies of biofilm formation by bacteria are crucial for understanding bacterial resistance and for development of novel antibacterial strategies. We have developed a new bioluminescence biofilm assay for Listeria innocua, which is considered a non-pathogenic surrogate for Listeria monocytogenes. L. innocua was transformed with a plasmid for inducible expression of NanoLuc luciferase (Nluc). Concentration-dependent bioluminescence signals were obtained over a concentration range of more than three log units. This biofilm assay enables absolute quantification of bacterial cells, with the necessary validation. For biofilm detection and quantification, this "Nluc bioluminescence" method has sensitivity of 1.0 × 10⁴ and 3.0 × 10⁴ colony forming units (CFU)/m

SUBMITTER: Berlec A 

PROVIDER: S-EPMC7901905 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature