EGFR-specific single-chain variable fragment antibody-conjugated Fe₃O₄/Au nanoparticles as an active MRI contrast agent for NSCLC.

ABSTRACT: Overexpression of epidermal growth factor receptor (EGFR) is closely associated with a poor prognosis in non-small cell lung cancer (NSCLC), thus making it a promising biomarker for NSCLC diagnosis. Here, we conjugated a single-chain antibody (scFv) targeting EGFR with Fe₃O₄/Au nanoparticles to form an EGFR-specific molecular MRI bioprobe (scFv@Fe₃O₄/Au) to better detect EGFR-positive NSCLC tumors in vivo. In vitro, we demonstrated that the EGFR-specific scFv could specifically deliver Fe₃O₄/Au to EGFR-positive NSCLC cells. In vivo experiments showed that the accumulation of scFv@Fe₃O₄/Au in tumor tissue was detectable by magnetic resonance imaging (MRI) at the indicated time points after systemic injec

SUBMITTER: Lu Y 

PROVIDER: S-EPMC7902179 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature