Optimization of adeno-associated viral vector-mediated transduction of the corticospinal tract: comparison of four promoters.

ABSTRACT: Adeno-associated viral vectors are widely used as vehicles for gene transfer to the nervous system. The promoter and viral vector serotype are two key factors that determine the expression dynamics of the transgene. A previous comparative study has demonstrated that AAV1 displays efficient transduction of layer V corticospinal neurons, but the optimal promoter for transgene expression in corticospinal neurons has not been determined yet. In this paper, we report a side-by-side comparison between four commonly used promoters: the short CMV early enhancer/chicken β actin (sCAG), human cytomegalovirus (hCMV), mouse phosphoglycerate kinase (mPGK) and human synapsin (hSYN) promoter. Reporter constructs with each of these promoters were packaged in AAV1, and were injected in the sensorimotor cor

SUBMITTER: Nieuwenhuis B 

PROVIDER: S-EPMC7902269 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature