A role for calcium-dependent protein kinases in differential CO₂ - and ABA-controlled stomatal closing and low CO₂ -induced stomatal opening in Arabidopsis.

ABSTRACT: Low concentrations of CO₂ cause stomatal opening, whereas [CO₂ ] elevation leads to stomatal closure. Classical studies have suggested a role for Ca²⁺ and protein phosphorylation in CO₂ -induced stomatal closing. Calcium-dependent protein kinases (CPKs) and calcineurin-B-like proteins (CBLs) can sense and translate cytosolic elevation of the second messenger Ca²⁺ into specific phosphorylation events. However, Ca²⁺ -binding proteins that function in the stomatal CO₂ response remain unknown. Time-resolved stomatal conductance measurements using intact plants, and guard cell patch-clamp experiments were performed. We isolated cpk quintuple mutants and analyzed stomatal movements in response to CO₂ , light

SUBMITTER: Schulze S 

PROVIDER: S-EPMC7902375 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature