Skeletal muscle myosin and cardiac myosin attenuate heparin's antithrombin-dependent anticoagulant activity.

ABSTRACT:

Background

Heparin enhances the ability of the plasma protease inhibitor, antithrombin, to neutralize coagulation factor Xa and thrombin. Skeletal muscle myosin binds unfractionated heparin.

Objectives

The aim of this study was to investigate the influence of myosin binding to heparin on antithrombin's anticoagulant activity.

Methods

Inhibition of factor Xa and thrombin by antithrombin in the presence of different heparins and skeletal muscle myosin or cardiac myosin was studied by measuring inhibition of each enzyme's chromogenic substrate hydrolysis.

Results and conclusions

Skeletal muscle myosin and cardiac myosin neutralized unfractionated heparin's enhancement of antithrombin's inhibition of purified factor Xa and thrombin. Skeletal muscle myosin also redu

SUBMITTER: Morla S 

PROVIDER: S-EPMC7902397 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature