In Vitro and In Vivo Sequestration of Phencyclidine by Me₄ Cucurbit[8]uril*.

ABSTRACT: We report investigations of the use of cucurbit[8]uril (CB[8]) macrocycles as an antidote to counteract the in vivo biological effects of phencyclidine. We investigate the binding of CB[8] and its derivative Me₄ CB[8] toward ten drugs of abuse (3-9, 12-14) by a combination of ¹ H NMR spectroscopy and isothermal titration calorimetry in phosphate buffered water. We find that the cavity of CB[8] and Me₄ CB[8] are able to encapsulate the 1-amino-1-aryl-cyclohexane ring system of phencyclidine (PCP) and ketamine as well as the morphinan skeleton of morphine and hydromorphone with K_d values ≤50 nm. In vitro cytotoxicity (MTS metabolic and adenylate kinase cell death assays in HEK293 and HEPG2 cells) and in vivo maximum tolerated dose studies (Swiss We

SUBMITTER: Murkli S 

PROVIDER: S-EPMC7902406 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature