Platelet-conditioned media induces an anti-inflammatory macrophage phenotype through EP4.

ABSTRACT:

Background

Platelets are increasingly recognized as immune cells. As such, they are commonly seen to induce and perpetuate inflammation; however, anti-inflammatory activities are increasingly attributed to them. Atherosclerosis is a chronic inflammatory condition. Similar to other inflammatory conditions, the resolution of atherosclerosis requires a shift in macrophages to an M2 phenotype, enhancing their efferocytosis and cholesterol efflux capabilities.

Objectives

To assess the effect of platelets on macrophage phenotype.

Methods

In several in vitro models employing murine (RAW264.7 and bone marrow-derived macrophages) and human (THP-1 and monocyte-derived macrophages) cells, we exposed macrophages to media in which non-agonized human platelets were cultured for 60 

SUBMITTER: Heffron SP 

PROVIDER: S-EPMC7902474 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature