The influence of Al³⁺ on DNA methylation and sequence changes in the triticale (× Triticosecale Wittmack) genome.

ABSTRACT: Abiotic stressors such as drought, salinity, and exposure to heavy metals can induce epigenetic changes in plants. In this study, liquid chromatography (RP-HPLC), methylation amplified fragment length polymorphisms (metAFLP), and methylation-sensitive amplification polymorphisms (MSAP) analysis was used to investigate the effects of aluminum (Al) stress on DNA methylation levels in the crop species triticale. RP-HPLC, but not metAFLP or MSAP, revealed significant differences in methylation between Al-tolerant (T) and non-tolerant (NT) triticale lines. The direction of methylation change was dependent on phenotype and organ. Al treatment increased the level of global DNA methylation in roots of T lines by approximately 0.6%, whereas demethylation of approximately 1.0% was observed in NT lin

SUBMITTER: Niedziela A 

PROVIDER: S-EPMC7902597 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature