Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca²⁺ levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia.

ABSTRACT: Benign prostatic hyperplasia (BPH) with lower urinary tract symptoms (LUTS) is a common disease among elderly men, for which safe and effective treatment strategies remain limited. The aim of the present study was to explore the potential effects of phosphodiesterase 5A3 (PDE5A3) silencing on human prostate smooth muscle cells (HPSMCs). HPSMCs were initially obtained from patients with BPH/LUTS. Short hairpin RNA (shRNA) targeting the PDE5A3 gene was subsequently transfected into cultured HPSMCs. The expression of PDE5A3 was measured using reverse transcription-quantitative PCR and western blotting. cGMP levels were then measured using western blotting and immunocytochemical staining and intracellular Ca²⁺ concentration was measured using rhod2-AM in HPSMCs after transfection. H

SUBMITTER: Xu Z 

PROVIDER: S-EPMC7903389 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature