Ontology highlight
ABSTRACT:
SUBMITTER: Gamboa CM
PROVIDER: S-EPMC7903462 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
STAR protocols 20210218 1
Robust patient-derived platforms that recapitulate the cellular and molecular fingerprints of glioblastoma are crucial for developing effective therapies. Here, we describe a chemically defined protocol for 3D culture and propagation of glioblastoma in 3D gliospheres, patient-derived organoids (PDOs), mouse brain orthotopic xenografts (PDOXs), and downstream drug and immunofluorescence assays. This simple-to-follow protocol allows assessing drug sensitivity, on-target activity, and combined drug ...[more]