Chronic inflammation of middle ear cholesteatoma promotes its recurrence via a paracrine mechanism.

ABSTRACT:

Background

Cholesteatoma disease is an expanding lesion in the middle ear. Hearing loss and facial paralysis alongside with other intracranial complications are found. No pharmaceutical treatment is available today and recurrence after surgical extraction occurs. We investigated possible TLR4-based mechanisms promoting recurrence and explore possible treatments strategies.

Methods

We isolated fibroblasts and epidermal stem cells from cholesteatoma tissue and healthy auditory canal skin. Subsequently, their expression under standard culture conditions and after stimulation with LPS was investigated by RT-qPCR. Cell metabolism and proliferation were analysed upon LPS treatment, with and without TLR4 antagonist. An indirect co-culture of fibroblasts and epidermal stem cells iso

SUBMITTER: Schurmann M 

PROVIDER: S-EPMC7903614 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature