Age-dependent decrease in TRPM4 channel expression but not trafficking alters urinary bladder smooth muscle contractility.

ABSTRACT: During development, maturation, or aging, the expression and function of urinary bladder smooth muscle (UBSM) ion channels can change, thus affecting micturition. Increasing evidence supports a novel role of transient receptor potential melastatin-4 (TRPM4) channels in UBSM physiology. However, it remains unknown whether the functional expression of these key regulatory channels fluctuates in UBSM over different life stages. Here, we examined TRPM4 channel protein expression (Western blot) and the effects of TRPM4 channel inhibitors, 9-phenanthrol and glibenclamide, on phasic contractions of UBSM isolated strips obtained from juvenile (UBSM-J, 5-9 weeks old) and adult (UBSM-A, 6-18 months old) male guinea pigs. Compared to UBSM-J, UBSM-A displayed a 50-70% reduction in total TRPM4 protein

SUBMITTER: Maxwell SE 

PROVIDER: S-EPMC7903938 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature