Single-cell sequencing reveals the potential oncogenic expression atlas of human iPSC-derived cardiomyocytes.

ABSTRACT: Human induced pluripotent stem cells (iPSCs) are important source for regenerative medicine. However, the links between pluripotency and oncogenic transformation raise safety issues. To understand the characteristics of iPSC-derived cells at single-cell resolution, we directly reprogrammed two human iPSC lines into cardiomyocytes and collected cells from four time points during cardiac differentiation for single-cell sequencing. We captured 32,365 cells and identified five molecularly distinct clusters that aligned well with our reconstructed differentiation trajectory. We discovered a set of dynamic expression events related to the upregulation of oncogenes and the decreasing expression of tumor suppressor genes during cardiac differentiation, which were similar to the gain-of-function an

SUBMITTER: Ou M 

PROVIDER: S-EPMC7903994 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature