Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku.

ABSTRACT: Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.

SUBMITTER: Jones CE 

PROVIDER: S-EPMC7904001 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature