Mitochondrial Transcription Factor A Binds to and Promotes Mutagenic Transcriptional Bypass of O⁴-Alkylthymidine Lesions.

ABSTRACT: O²- and O⁴-alkylated thymidine lesions are known to be poorly repaired and persist in mammalian tissues. To understand how mammalian cells sense the presence and regulate the repair of these lesions, we employed a quantitative proteomic method to discover regioisomeric O²- and O⁴-n-butylthymidine (O²- and O⁴-nBudT)-binding proteins. We were able to identify 21 and 74 candidate DNA damage recognition proteins for O²-nBudT- and O⁴-nBudT-bearing DNA probes, respectively. Among these proteins, DDB1 and DDB2 selectively bind to O²-nBudT-containing DNA, whereas three high-mobility group (HMG) proteins

SUBMITTER: He X 

PROVIDER: S-EPMC7904241 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature