Polysialic acid and Siglec-E orchestrate negative feedback regulation of microglia activation.

ABSTRACT: Polysialic acid (polySia) emerges as a novel regulator of microglia activity. We recently identified polysialylated proteins in the Golgi compartment of murine microglia that are released in response to inflammatory stimulation. Since exogenously added polySia is able to attenuate the inflammatory response, we proposed that the release of polysialylated proteins constitutes a mechanism for negative feedback regulation of microglia activation. Here, we demonstrate that translocation of polySia from the Golgi to the cell surface can be induced by calcium depletion of the Golgi compartment and that polysialylated proteins are continuously released for at least 24 h after the onset of inflammatory stimulation. The latter was unexpected, because polySia signals detected by immunocytochemistry a

SUBMITTER: Thiesler H 

PROVIDER: S-EPMC7904730 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature