Multiple Displacement Amplification as a Solution for Low Copy Number Plasmid Sequencing.

ABSTRACT: Plasmids play a major role in bacterial adaptation to environmental stress and often contribute to antibiotic resistance and disease virulence. Although the complete sequence of each plasmid is essential for studying plasmid biology, most antibiotic resistance and virulence plasmids in Salmonella are present only in a low copy number, making extraction and sequencing difficult. Long read sequencing technologies require higher concentrations of DNA to provide optimal results. To resolve this problem, we assessed the sufficiency of multiple displacement amplification (MDA) for replicating Salmonella plasmid DNA to a satisfactory concentration for accurate sequencing and multiplexing. Nine Salmonella enterica isolates, representing nine different serovars carrying plasmid

SUBMITTER: Yao K 

PROVIDER: S-EPMC7904871 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature