Verteporfin inhibits the persistent fibrotic phenotype of lesional scleroderma dermal fibroblasts.

ABSTRACT: Fibrosis is perpetuated by an autocrine, pro-adhesive signaling loop maintained by the synthetic and contractile abilities of myofibroblasts and the stiff, highly-crosslinked extracellular matrix. Transcriptional complexes that are exquisitely responsive to mechanotransduction include the co-activator YAP1, which regulates the expression of members of the CCN family of matricellular proteins such as CCN2 and CCN1. Although selective YAP1 inhibitors exist, the effect of these inhibitors on profibrotic gene expression in fibroblasts is largely unknown, and is the subject of our current study. Herein, we use genome-wide expression profiling, real-time polymerase chain reaction and Western blot analyses, cell migration and collagen gel contraction assays to assess the ability of a selective YA

SUBMITTER: Shi-Wen X 

PROVIDER: S-EPMC7905010 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature