Isolation and Multiple Differentiation of Rat Pericardial Fluid Cells.

ABSTRACT:

Objective

The aim of the present study is to isolate and analyze the characterization of pericardial fluid cells (PFCs) from rat and provides a morphological basis for the basic research and clinical application of PFCs.

Methods

After aseptic thoracotomy was performed, normal saline was injected into the pericardial cavity of 50 adult Sprague-Dawley rats. The mixture of diluted pericardial fluid was extracted, centrifuged, and cultured. The cell morphology of different generations in the pericardial fluid was observed on an inverted microscope. The expression levels of CD44, CD29, CD90, and pan-hematopoietic marker CD45 were analyzed via flow cytometry. The third-generation cells were used for osteogenic, adipogenic, and cardiac differentiation.

Results

PFCs were succ

SUBMITTER: Sun Y 

PROVIDER: S-EPMC7905039 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature