Notch intracellular domain regulates glioblastoma proliferation through the Notch1 signaling pathway.

ABSTRACT: Notch intracellular domain (NICD), also known as the activated form of Notch1 is closely associated with cell differentiation and tumor invasion. However, the role of NICD in glioblastoma (GBM) proliferation and the underlying regulatory mechanism remains unclear. The present study aimed to investigate the expression of NICD and Notch1 downstream gene HES5 in human GBM and normal brain samples and to further detect the effect of NICD on human GBM cell proliferation. For this purpose, western blotting and immunohistochemical staining were performed to analyze the expression of NICD in human GBM tissues, while western blotting and reverse-transcription quantitative PCR experiments were used to analyze the expression of Hes5 in human GBM tissues. A Flag-NICD vector was used to overexpress NIC

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7905607 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature