Single-cell transcriptomic profiling and characterization of endothelial progenitor cells: new approach for finding novel markers.

ABSTRACT:

Background

Endothelial progenitor cells (EPCs) are promising candidates for the cellular therapy of peripheral arterial and cardiovascular diseases. However, hitherto there is no specific marker(s) defining precisely EPCs. Herein, we are proposing a new in silico approach for finding novel EPC markers.

Methods

We assembled five groups of chosen EPC-related genes/factors using PubMed literature and Gene Ontology databases. This shortened database of EPC factors was fed into publically published transcriptome matrix to compare their expression between endothelial colony-forming cells (ECFCs), HUVECs, and two adult endothelial cell types (ECs) from the skin and adipose tissue. Further, the database was used for functional enrichment on Mouse Phenotype database and protein-prote

SUBMITTER: Abdelgawad ME 

PROVIDER: S-EPMC7905656 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature