Assessing the ocular surface microbiome in severe ocular surface diseases.

ABSTRACT:

Purpose

There is growing evidence for a critical role of the microbiome in ocular health and disease. We performed a prospective, observational study to characterize the ocular surface microbiome (OSM) in four chronic ocular surface diseases (OSDs) and healthy controls.

Methods

Sterile swabs were used to collect samples from each eye of 39 patients (78 eyes). Sterile technique and multiple controls were used to assess contamination during DNA extraction, amplification and sequencing. Concurrent use of topical antibiotics, steroids, and bandage contact lenses (BCLs) was documented.

Results

Despite the low biomass of the ocular surface, 47/78 (60%) eyes sampled had positive sequencing reads. We observed that half of patients (8/17, 47%) had distinct microbiomes in each

SUBMITTER: Zilliox MJ 

PROVIDER: S-EPMC7905829 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature