Dual Channel Microfluidics for Mimicking the Blood-Brain Barrier.

ABSTRACT: High-resolution imaging is essential for analysis of the steps and way stations of cargo transport in in vitro models of the endothelium. In this study, we demonstrate a microfluidic system consisting of two channels horizontally separated by a cell-growth-promoting membrane. Its design allows for high-resolution (down to single-molecule level) imaging using a high numerical aperture objective with a short working distance. To reduce optical aberrations and enable single-molecule-sensitive imaging, an observation window was constructed in the membrane via laser cutting with subsequent structuring using 3D multiphoton lithography for improved cell growth. The upper channel was loaded with endothelial cells under flow conditions, which showed polarization and junction formation

SUBMITTER: Buchroithner B 

PROVIDER: S-EPMC7905877 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature