Establishing and evaluation of a polymerase chain reaction for the detection of Echinococcus multilocularis in human tissue.

ABSTRACT:

Background

Alveolar echinococcosis (AE) is caused by metacestode larva of the tapeworm Echinococcus multilocularis. AE diagnostics currently rely on imaging techniques supported by serology, but unequivocal detection of AE is difficult. Although polymerase chain reaction (PCR)-based methods to detect tapeworm DNA in biopsies have been suggested for several species, no validated protocol adhering to accepted guidelines has so far been presented for AE diagnostics. We herein established a PCR protocol for metacestode biopsies and technically evaluated the method using isolated parasite DNA and cells, biopsies of clinically relevant material, and formalin fixed paraffin-embedded (FFPE) human tissue blocks. We compared the results with an immunochemical (IHC) approach using the monoclo

SUBMITTER: Grimm J 

PROVIDER: S-EPMC7906421 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature