Transcription-dependent radial distribution of TCF7L2 regulated genes in chromosome territories.

ABSTRACT: Human chromosomes occupy distinct territories in the interphase nucleus. Such chromosome territories (CTs) are positioned according to gene density. Gene-rich CTs are generally located in the center of the nucleus, while gene-poor CTs are positioned more towards the nuclear periphery. However, the association between gene expression levels and the radial positioning of genes within the CT is still under debate. In the present study, we performed three-dimensional fluorescence in situ hybridization experiments in the colorectal cancer cell lines DLD-1 and LoVo using whole chromosome painting probes for chromosomes 8 and 11 and BAC clones targeting four genes with different expression levels assessed by gene expression arrays and RT-PCR. Our results confirmed that the two over-expressed gene

SUBMITTER: Torabi K 

PROVIDER: S-EPMC7906436 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature