Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis.

ABSTRACT: Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with

SUBMITTER: Hofemeier AD 

PROVIDER: S-EPMC7906603 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature