The Card1 nuclease provides defence during type III CRISPR immunity.

ABSTRACT: In the type III CRISPR-Cas immune response of prokaryotes, infection triggers the production of cyclic oligoadenylates that bind and activate proteins that contain a CARF domain^1,2. Many type III loci are associated with proteins in which the CRISPR-associated Rossman fold (CARF) domain is fused to a restriction  endonuclease-like domain^3,4. However, with the exception of the well-characterized Csm6 and Csx1 ribonucleases^5,6, whether and how these inducible effectors provide defence is not known. Here we investigated a type III CRISPR accessory protein, which we name cyclic-oligoadenylate-activated single-stranded ribonuclease and single-stranded deoxyribonuclease 1 (Card1). Card1 forms a symmetrical dimer that has a large central cavity between its CRISPR-

SUBMITTER: Rostol JT 

PROVIDER: S-EPMC7906951 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature