Optimized Culture Conditions for Improved Growth and Functional Differentiation of Mouse and Human Colon Organoids.

ABSTRACT:

Background & aims

Diligent side-by-side comparisons of how different methodologies affect growth efficiency and quality of intestinal colonoids have not been performed leaving a gap in our current knowledge. Here, we summarize our efforts to optimize culture conditions for improved growth and functional differentiation of mouse and human colon organoids.

Methods

Mouse and human colon organoids were grown in four different media. Media-dependent long-term growth was measured by quantifying surviving organoids via imaging and a cell viability readout over five passages. The impact of diverse media on differentiation was assessed by quantifying the number of epithelial cell types using markers for enterocytes, stem cells, Goblet cells, and enteroendocrine cells by qPCR a

SUBMITTER: Wilson SS 

PROVIDER: S-EPMC7906999 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature