An NKX2-1^GFP and TP63^tdTomato dual fluorescent reporter for the investigation of human lung basal cell biology.

ABSTRACT: Basal cells are multipotent stem cells responsible for the repair and regeneration of all the epithelial cell types present in the proximal lung. In mice, the elusive origins of basal cells and their contribution to lung development were recently revealed by high-resolution, lineage tracing studies. It however remains unclear if human basal cells originate and participate in lung development in a similar fashion, particularly with mounting evidence for significant species-specific differences in this process. To address this outstanding question, in the last several years differentiation protocols incorporating human pluripotent stem cells (hPSC) have been developed to produce human basal cells in vitro with varying efficiencies. To facilitate this endeavour, we introduced tdTomato into th

SUBMITTER: Goh KJ 

PROVIDER: S-EPMC7907081 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature