A bladder cancer patient-derived xenograft displays aggressive growth dynamics in vivo and in organoid culture.

ABSTRACT: Bladder cancer is among the most prevalent cancers worldwide. Currently, few bladder cancer models have undergone thorough characterization to assess their fidelity to patient tumors, especially upon propagation in the laboratory. Here, we establish and molecularly characterize CoCaB 1, an aggressive cisplatin-resistant muscle-invasive bladder cancer patient-derived xenograft (PDX) and companion organoid system. CoCaB 1 was a subcutaneous PDX model reliably transplanted in vivo and demonstrated an acceleration in growth upon serial transplantation, which was reflected in organoid and 2D cell culture systems. Transcriptome analysis revealed progression towards an increasingly proliferative and stem-like expression profile. Gene expression differences between organoid and PDX models reflecte

SUBMITTER: Cai EY 

PROVIDER: S-EPMC7907272 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature