Paternal low protein diet and the supplementation of methyl-donors impact fetal growth and placental development in mice.

ABSTRACT:

Introduction

Paternal low-protein diet can alter sperm methylation status, fetal growth and program offspring ill-health, however its impact on the placenta remains poorly defined. Here we examine the influence paternal low-protein diet has on fetal and placental development and the additional impact of supplementary methyl-donors on fetoplacental physiology.

Methods

Male C57BL/6J mice were fed a control normal protein diet (NPD; 18% protein), a low-protein diet (LPD; 9% protein) or LPD with methyl-donor supplementation (MD-LPD; choline chloride, betaine, methionine, folic acid, vitamin B12) for a minimum of 8 weeks. Males were mated with 8-11 week old female C57BL/6J mice and fetal and placental tissue collected on embryonic day 17.5.

Results

Paternal LPD was associa

SUBMITTER: Morgan HL 

PROVIDER: S-EPMC7907633 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature