The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone.

ABSTRACT:

Background

Cholesterol gallstone (CG) is the most common gallstone disease, which is induced by biliary cholesterol supersaturation. The purpose of this study is to investigate the pathogenesis of CG.

Methods

Sixteen mice were equally and randomly divided into model group and normal control group. The model group was fed with lithogenic diets to induce CG, and then gallbladder bile lipid analysis was performed. After RNA-seq library was constructed, differentially expressed mRNAs (DE-mRNAs) and differentially expressed lncRNAs (DE-lncRNAs) between model group and normal control group were analyzed by DESeq2 package. Using the cluster Profiler package, enrichment analysis for the DE-mRNAs was carried out. Based on Cytoscape software, the protein-protein interaction (PPI) netw

SUBMITTER: Qian C 

PROVIDER: S-EPMC7908887 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature